HvWRKY2 acts as an immunity suppressor and targets HvCEBiP to regulate powdery mildew resistance in barley
| 论文作者 | Deshui Yu*, Renchun Fan, Ling Zhang, Pengya Xue, Libing Liao, Meizhen Hub, Yanjun Cheng,Jine Li, Ting Qi, Shaojuan Jing, Qiuyun Wang, Arvind Bhatt, Qian-Hua Shen* |
| 刊物 | The Crop Journal |
| 标识符 | 10.1016/j.cj.2022.05.010 |
| 摘要 | Plants use a sophisticated immune system to perceive pathogen infection and activate immune responses in a tightly controlled manner. In barley, HvWRKY2 acts as a repressor in barley disease resistance to the powdery mildew fungus, Blumeria graminis f. sp. hordei (Bgh). However, the molecular features of HvWRKY2 in its DNA-binding and repressor functions, as well as its target genes, are uncharacterized.We show that the W-box binding of HvWRKY2 requires an intact WRKY domain and an upstream sequence of 75 amino acids, and the HvWRKY2 W-box binding activity is linked to its repressor function in disease resistance. Chromatin immunoprecipitation (ChIP)-seq analysis identified HvCEBiP, a putative chitin receptor gene, as a target gene of HvWRKY2 in overexpressing transgenic barley plants. ChIP-qPCR and Electrophoretic Mobility Shift Assay (EMSA) verified the direct binding of HvWRKY2 to a W-boxcontaining sequence in the HvCEBiP promoter. HvCEBiP positively regulates resistance against Bgh in barley. Our findings suggest that HvWRKY2 represses barley basal immunity by directly targeting pathogen-associated molecular pattern (PAMP) recognition receptor genes, suggesting that HvCEBiP and likely chitin signaling function in barley PAMP-triggered immune responses to Bgh infection. |
