Light-induced Dof transcription factors up-regulate the andrographolide biosynthesis in Andrographis paniculata
| 作者 | Xingbin Lv a,b,1 , Hua Yang a,1 , Yufang Hu a, Tingting Jing a,d , Ying Xiong a , Qi Liang a,c,Shuyun Tian a , Lang Yang a , Zhiyi Zhang a,c , Mingkun Huang a,c , Yanqin Xu b ,Ling Zhang a,c,* |
| 刊物 | Industrial Crops & Products |
| 标识符 | 10.1016/j.indcrop.2025.121715 |
| 摘要 | Andrographis paniculata (A. paniculata) is a traditional Chinese medicinal plant that accumulates andrographolide,a key bioactive diterpenoid compound. In this study, we found that light can upregulate the production of andrographolide. However, the underlying regulatory mechanisms governing its biosynthesis remain elusive. By coupling the RNA-seq data obtained under different light conditions with co-expression analysis, two candidate transcription factors, ApDof16 and ApDof29, were identified. Using virus-induced gene silencing (VIGS), we demonstrated that these two Dofs were able to regulate the content of andrographolide as well as the expression of ApCPS2, a key gene of the andrographolide biosynthesis pathway. Further experiments, such as the yeast onehybrid (Y1H) assays, transient assays and chromatin immunoprecipitation quantitative PCR (ChIP-qPCR) analysis indicated that ApDof29 rather than ApDof16, directly binds to the promoter of ApCPS2, thereby regulating its expression and the subsequent andrographolide accumulation in response to light. |
