A review of the advances and perspectives in sequencing technologies for analysing plant epigenetic responses to abiotic stress
| 作者 | Siqing Fan , Hua Yang , Yufang Hu , Ling Zhang *, Mingkun Huang * |
| 刊物 | Plant Stress |
| 标识符 | 10.1016/j.stress.2025.101144 |
| 摘要 | Abiotic stresses, such as drought, salinity, heat, and cold constrain plant growth and productivity by influence plant internal regulatory networks. Transcriptional/epigenetic regulation, which encompasses mechanisms such as DNA methylation, histone modifications, chromatin accessibility, non-coding RNAs, and RNA modifications, orchestrates rapid transcriptional reprogramming and stress memory and provides key adaptive capacity for plants to resist stress. Recent sequencing breakthrough system-level mapping of these layers, including ATAC-seq (accessibility), CUT&Tag/ChIP-seq (histone marks), Hi-C (3D genome), WGBS (methylomes), lncRNA/small-RNA profiling (regulatory RNAs), and Nanopore direct RNA sequencing (RNA modification). This review summarizes the application of these methods to capture the landscape of dynamic DNA methylation, chromatin conformation changes, non-coding RNA regulation, and RNA modification under abiotic stress conditions, andaddresses current technical challenges in multi-omics research and explores future perspectives. |
