Telomere-to-telomere genome assembly and multi-omics analyses illustrate the high accumulation of quercetin glucosides in tetraploid Descurainia sophia
| 作者 | Weifeng Wu1,3,8 ,Jianyong Wang2,3,8, Chengcheng Cai4,8, Xiaoyu Song5,8, Hua Li2,3,Tao Zhang2,3,Meixin Xiong2,3, Ying Wang6, Jie Zhang6 ,Bingbing Li7 ,Lei Zhang4, Feng Li7, Mingkun Huang3，Wei Li5, Feng Cheng4,*, Danyu Kong1,3,*, and Yi Liu2,3,* |
| 刊物 | Horticulture Research |
| 标识符 | 10.1093/hr/uhaf335 |
| 摘要 | Quercetin glucosides are important phytopharmaceutical metabolites in Descurainia sophia seeds, which are widely used in traditional herbal medicine. However, the key genes involved in quercetin glucoside biosynthesis in D. sophia have not been characterized. Herein, we present the telomere-to-telomere genomes of a tetraploid D. sophia, which accumulates high levels of quercetin glucoside, and a diploid D. sophia, which accumulates only trace amounts. Multi-omics analyses and uridine diphosphate glucosyltransferase (UGT) enzyme assays revealed that the gene duplication and functional evolution of Dscd6AG01520, a UGT gene, led to high quercetin-3-O-β-D-glucoside and quercetin-3,7-O-β-D-diglucoside accumulation in tetraploid D. sophia seeds. Further UGT enzyme assays with the point mutations of Dscd6AG01520 showed that S213 was a critical amino acid for the enzymatic activity of Dscd6AG01520. In addition, we found that diploid D. sophia evolved from an ancestral crucifer karyotype through chromosome fusion and rearrangement. Collectively, our findings illuminate the mechanism of high quercetin glucoside accumulation in tetraploid D. sophia, clarify the origin of the diploid D. sophia genome, and provide valuable genomic resources for comparative genomics and research into polyploid evolution. |
